The interest in education for entrepreneurship in primary and secondary schools constantly rises and it arouses a need to examine its impact on certain entrepreneurial characteristics. Not much research focusing on this topic has been conducted as yet. Research usually shows its positive impact on the intention to do business. In this study, we examined the mutual relation of five variables: pupils’ entrepreneurial spirit, pupils’ attitude to entrepreneurship, the school education focus on entrepreneurship, teachers’ influence and the influence of their relatives-entrepreneurs. To collect data, we used ASTEE questionnaire. The researched sample consisted of 268 pupils from primary and secondary schools in the Czech Republic. Only medium or small correlation values were found between the researched variables, which means that the formation of pupils’ entrepreneurial spirit and attitude to entrepreneurship is not principally influenced by the learning content about entrepreneurship and by teachers. The findings can support the opinion that education for entrepreneurship and preparation for entrepreneurship is more effective in a thought-out entrepreneurial ecosystem.