Under the current conditions of expanding globalization, the role of cross-border cooperation in the life of regions and communities is growing and new opportunities are opening up to intensify economic activity in peripheral areas and increase their competitiveness. Cross-border cooperation is aimed at overcoming the negative aspects of the existence of borders and the consequences that have arisen in the border areas due to their location on the national outskirts of states and aims to improve living conditions. Among the main goals of such cooperation are: overcoming existing stereotypes and prejudices on both sides of the border; removing political and administrative barriers between neighboring nations; creation of economic, social and cultural infrastructure, subject to the formation of joint bodies, economic entities, centers, etc. The article describes the main aspects of cross-border cooperation between Romania, Moldova and Ukraine. Romania is an important partner for the countries of Eastern Europe and, as one of the youngest EU countries, has considerable experience that could help partner countries to carry out effective socio- economic reforms and fight corruption. Ukraine-Romania- Moldova cross-border cooperation is part of the European Neighborhood and Partnership Instrument and plays an important role in developing mutually beneficial relations between states, addressing socio-economic, environmental and security issues, and is an effective means of Ukraine’s European integration. The Joint Action Plan “Ukraine-Romania- Moldova” 2007–2013 of the European Instrument has significantly affected the revival of cross-border cooperation between Ukraine, Romania, Moldova, especially the cooperation of local and regional authorities, communities and civil society institutions. During the program, more than 140 projects were implemented, which contributed to the development of transport infrastructure in border areas, energy networks, water and waste management, funded projects for cooperation between universities, research institutes and local authorities. There are positive dynamics of cross-border cooperation between Ukraine and Romania, in particular, important infrastructure projects are being implemented under the “Joint Black Sea Basin Joint Operational Program 2014–2020” and EU Joint Operational Program Romania-Ukraine 2014–2020”.