After the beginning of the conflict in Ukraine and Russia’s annexation of the Crimean Peninsula, the Czech Republic became fully aware of the threats posed by the Kremlin despite President Zeman has denied the presence of Russian troops in Ukraine and has criticised the EU sanctions against Russia. Czechia belongs to the group of countries through which Russia influences the EU, to gradually and deliberately erode its structures. Russia exerts a strong influence on the Czech Republic by non-military means, including disinformation and propaganda, the activities of secret services, and penetration of its economy and specifically its energy sector. The article aims to answer the question about the role of Russian disinformation and propaganda in the context of Russian influence in the Czech Republic. The role of Russian disinformation and propaganda and how Russia influences Czechia is extensively analysed. The main hypothesis is that Russia treats the Czech Republic as a key state for espionage and disinformation activities and as a zone of influence, undermining the sovereignty of the Czech Republic and the role of NATO and the EU.