The contribution focuses on the investigation into the influence of gender on students’ achievement motivation. Achievement motivation perceived as a relatively stable predisposition of an individual is an important factor of achievement. The research was performed on the sample of 213 university students, out of whom 102 were women and 111 men. The research method was achievement motivation inventory (AMI), which diagnoses the achievement motive, anxiety hindering achievement and anxiety supporting achievement. The research findings have confirmed assumptions about gender differences in all the measured variables.