Poland is the biggest FDI recipient of the EU countries located in Central Europe. The statistics are showing also more and more investment high activity of Polish companies on the foreign markets. Towards the strong competition and high costs of the workforce in EU countries, Polish companies as the location of their investments often choose markets outside the EU, including the Russian market. The article presents the analysis of the investment cooperation between Poland and the Russian Federation and shows the essential barriers negatively influencing in the shape of these relations. He is pointing in addition also the high importance of the role of the economic diplomacy in the development of cooperation investment between both countries.